Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae-bacteria that inhabit the gastrointestinal tract.
Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
Applications:Suitable for use in ELISA, Dot Blot and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:goat
Source Antibody:E. coli
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Alkaline Phosphatase (AP).
Specificity:Recognizes all E. coli species in immunogen, plus O157-H7. Recognizes more than 50 proteins by Western Blot.
Isotype:IgG
Calc Applications Abbrev:DB E WB
Calc Crossreactivity:Ec
Immunogen:Heat inactivated blend of 6 different strains of E. coli: TOP 10F, HB101, JM109, BL21, DH5 alpha and K12.