84-2287-01　［取扱停止］Complement C1q, Human (AP) 100ul　C7850-06-AP

特徴

• C1q is serum glycoprotein of 18-polypeptides chains consisting of three non-identical subunits, A (29kD), B (26kD) and C (246aa, 19kD) in molar ratio of 1:2:2.
• C1Q in the plasma is complexed with two proenzymes C1r and two C1s molecules to form the first component of complement (C1).
• Activation of complement via classical pathway is triggered by binding of globular head of C1q to immune complexes containing IgG (Fc-region) or IgM or to a variety of other activating substances, including C-reactive protein, retrovirus and mitochondria.
• Subsequent to C1q binding, c1r and C1s are converted to proteolytic enzymes that are responsible to continuation of activation via the classical pathway.
• Normal plasma concentration of C1q is 100-180mg/L.
• Applications:Suitable for use in ELISA, RID, Double Diffusion, IEP and Rocket IEP.
• Other applications not tested.
• Recommended Dilution:RID: Neat-1:2 using 0.1ul antiserum/cm2 in gel vs 20ul fresh plasma
• Double Diffusion: 10ul antiserum vs 20ul fresh plasmaNote: The use of 3% PEG 6000 with 1.4% agarose in a suitable buffer such as Tris/Glycine/EDTA (6.1g/L Tris, 3.8g/L Glycine, 8.8g/L NaCl, 3.7g/L EDTA (pH 8.4)) is recommended
• Note: Results can be enhanced by washing completed agarose gels in saline, drying and finally staining with a protein stain such as coomassie blue
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C before opening.
• DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.
• Stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
• Note: Applications are based on unconjugated antibody.