Vancomycin is an aminoglycoside antibiotic, used in the treatment of infections caused by Gram-positive bacteria.
vancomycin is not active against Gram-negative bacteria (except some non-gonococcal species of Neisseria).
The main target of this antibiotic is the D-alanyl-D-alanine terminal dipeptide of peptidoglycan precursors, used by bacteria for constructing their cell walls.
This prevents the reaction used to link peptidoglycan precursors together from taking place.
Vancomycin binds with the substrate, not the enzyme, in contrast to the way penicillin inhibits peptidoglycan synthesis.
The large hydrophilic molecule is able to form hydrogen bond interactions with the terminal D-alanyl-D-alanine moieties of the NAM/NAG-peptides.
Under normal circumstances, this is a five-point interaction.
This binding of vancomycin to the D-Ala-D-Ala prevents cell wall synthesis in two ways.
It prevents the synthesis of the long polymers of N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) that form the backbone strands of the bacterial cell wall, and it prevents the backbone polymers that do manage to form from cross-linking with each other.
Applications:Suitable for use in Indirect FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Allophycocyanin (APC).
