It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus.
Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans.
Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb).
The first step in coronavirus infection is binding of the viral spike protein, a 139-kD protein, to certain receptors on host cells.
The spike protein is the main surface antigen of the coronavirus.
The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
Allophycocyanin (APC) is light sensitive.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes SARS Spike protein
Isotype：IgG
Calc Applications Abbrev：FLISA
Immunogen：Synthetic peptide corresponding to 14 amino acids at the amino terminal of the SARS Spike glycoprotein
