84-2285-37　AMACR (Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM, p504S) (FITC) 100ul　168645-AF-FITC

特徴

• This antibody recognizes a protein of 54kD, which is identified as AMACR, also known as p504S.
• It is an enzyme that is involved in bile acid biosynthesis and b-oxidation of branched-chain fatty acids.
• AMACR is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma.
• The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction.
• AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia.
• A spotty granular cytoplasmic staining is seen in a few cells of the benign glands.
• Applications: Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:100-1:200 for 2 hours at RTImmunohistochemistry: formalin-fixed: 1:50-1:100 for 30-60 minutes at RT(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutesOptimal dilutions to be determined by the researcher.
• Positive Control:HEK cells or Prostate Adenocarcinoma
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.