Uroplakin-3A (UPK3A) is a component of the asymmetric unit membrane (AUM).
It is a highly specialized bio-membrane made by terminally differentiated urothelial cells.
The protein may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells.
UPK3A also contributes to the formation of urothelial glycocalyx, which may play an important role in preventing bacterial adherence through FimH bacterial protein binding leading to bladder infection .
UPK3A has been shown as a helpful marker for the detection of bladder cancer.
Applications:Suitable for use in Western Blot, Flow Cytometry (Not Tested), Immunoprecipitation and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlFlow Cytometry (Not Tested): 0.5-1ug/10e6 cellsImmunofluorescence: 1-2ug/mlImmunoprecipitation: 1-2ug/500ug protein lysateOptimal dilutions to be determined by the researcher.
Positive Control:HEK2 93 cells or bladder carcinoma
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human UPK3A.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa260-279 (GASESSYTSVNRGPPLDRAE) from human UPK3A.
