Uroplakin-3A (UPK3A) is a component of the asymmetric unit membrane (AUM).
It is a highly specialized bio-membrane made by terminally differentiated urothelial cells.
The protein may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells.
UPK3A also contributes to the formation of urothelial glycocalyx, which may play an important role in preventing bacterial adherence through FimH bacterial protein binding leading to bladder infection .
UPK3A has been shown as a helpful marker for the detection of bladder cancer.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/ml0.5-1ug/10e6 cellsImmunofluorescence: 1-2ug/mlImmunoprecipitation: 1-2ug/500ug protein lysateOptimal dilutions to be determined by the researcher.
Positive Control:HEK2 93 cells or bladder carcinoma
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phophatase (AP).
Specificity：Recognizes human UPK3A.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa260-279 (GASESSYTSVNRGPPLDRAE) from human UPK3A.
