84-2285-18　p40 (p63 Delta) (Biotin) 100ul　138580-AF-Biotin

特徴

• p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application.
• The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors.
• The ability to differentiate between lung adenocarcinoma vs.
• squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment.
• p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40.
• The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.
• Applications:Suitable for use in Flow Cytometry (Not Tested), Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (FFPE): Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutesOptimal dilutions to be determined by the researcher.
• Positive Control:HEK293 cells or Prostate Carcinoma or Lung Squamous Cell Carcinoma
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.