84-2285-13　Napsin-A (FITC) 100ul　138575-AF-FITC

特徴

• Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.
• There are two closely related napsins, napsin A and napsin B.
• Napsin A is expressed as a single chain protein.
• Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen.
• Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung.
• The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
• Applications:Suitable for use in Flow Cytometry (Not Tested), Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry (Not Tested): 0.5-1ug/10e6 cellsImmunofluorescence: 1-2ug/mlWestern Blot: 0.5-1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RTImmunoprecipitation: 1-2ug/500ug protein lysateOptimal dilutions to be determined by the researcher.
• Positive Control:Lung adenocarcinoma
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.