84-2285-06　CD8a (T-cell Surface Glycoprotein CD8 alpha Chain) (Biotin) 100ul　138530-AF-Biotin

特徴

• CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha).
• A majority of thymocytes and a subpopulation of mature T cells and NK cell express CD8a.
• CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.
• For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4.
• It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-.
• Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed.
• CD8 is also found in littoral cell angioma of the spleen.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry (Not Tested) and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1-2ug/mlImmunoprecipitation: 1-2ug/500ug protein lysateFlow Cytometry (Not Tested): 1-2ug/10e6 cellsWestern Blot: 1-2ug/mlImmunohistochemistry (Frozen & Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RTOptimal dilutions to be determined by the researcher.
• Positive Control:HuT78 or hPBL.
• Tonsil
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.