The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR).
Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition.
The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage.
In cortical thymocytes, CD3 is predominantly intra-cytoplasmic.
However, in medullary thymocytes, it appears on the T cell surface.
CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Applications:Suitable for use in Western Blot, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control:Jurkat cells.
Tonsil and lymph node
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Specificity：Recognizes human CD3e. Recognizes the e-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kD. Species Crossreactivity: mouse. Species sequence homology: elephant, gibbon, guinea pig, hamster, equine, rat, monkey, mouse, orangutan, porine, rabbit, sheep, ape, feline, chicken, chimpanzee, bovine, canine, frog, opossum.
Isotype：IgG
Calc Applications Abbrev：FC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa156-168 of human CD3e (ERPPPVPNPDYEP). Cellular Localization: Cell surface and cytoplamic
