84-2281-13　［取扱停止］RBP3 (Interstitial Retinol Binding Protein 3, IRBP, RBPI, RP66, D10S64, D10S65, D10S66) 100ul　219189

特徴

• Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells.
• It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5kbp in length and consists of four exons separated by three introns.
• The introns are 1.6-1.9kbp long.
• The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000D.
• The aa sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events.
• In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected.
• The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long.
• The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and ~740 base pairs long, respectively.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:50-1:100Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.