Thymidylate kinase (DTYMK) catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.
DTYMK is essential for DNA synthesis and is an important intermediate enzyme in the pathway of many pyrimidine analog drugs, including 5-Fluorouracil (5-FU), the chemotherapeutic drug of choice for several solid tumors.
The human DTYMK gene was isolated via functional complementation of a Saccharomyces cerevisiae cell cycle mutant, cdc8.
DTYMK mRNA levels and enzyme activities are regulated at the level of cell cycle and cell growth pathways.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DTYMK.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human DTYMK.
