84-2272-45　［取扱停止］Complement Component 1R (C1r) 50ul　218885

特徴

• C1r is a plasma protein and forms part of the C1 pentamer, the first component of the classical complement pathway.
• The C1r dimer binds to two C1s molecules in a Ca2+ - dependent manner.
• This tetramer then binds to C1q to form the C1 pentamer.
• C1r is a serine protease and on the binding of C1to an immune complex, C1r self activates.
• Activated C1r (CTr) in turn activates C1s, the other serine protease component of C1.
• Activated C1 in turn activates C4 and C4bC2 and is inhibited by C1-inactivator.
• Normal plasma concentrations of C1r are around 34mg/L.
• Unactivated C1r consists of a single polypeptide chain of MW 173kD.
• When activated it is cleaved to two polypeptide chains linked by disulphide bonds, the A chain (55kD) and B chain (31kD).
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.