This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase.
The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome.
This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates.
This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation.
As such it functions as a negative regulator of many signal transduction pathways.
This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia.
Mutations in this gene are also the cause of Noonan syndrome-like disorder.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:20-1:100Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Phospho-CBL-Y700.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：A phospho specific peptide corresponding to the residues surrounding Y700 of human CBL.
