8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress.
There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease.
8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA.
Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation.
Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications.
In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
Industrial uses may extend to the dietary supplement manufacturers, who could benefit from an immunoassay that could be used to test the effectiveness of antioxidants and other nutraceuticals.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Pretreat with 10ug/ml of proteinase k for 40 minutes at 37°C prior to staining
Optimal dilutions to be determined by the researcher.
Positive Control: Skin, staining is limited to the nucleus.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.01% thimerosal.
Specificity：Recognizes human 8-Hydroxydeoxyguanosine. 100% inhibited by 8-hydroxydeoxyguanosine and 8-hydroxyguanosine nucleotides. Reacts with 8-mercaptoguanosine (80%), 8-bromoguanosine (5%). Does not react with 2-Deoxyadenosine, 7-methlyguanosine, guanosine monophosphate and guanosine.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：8-Hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine.
