The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4.
They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain.
VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells.
Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells.
KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling.
KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells.
Flt3 (also known as Flk-2) is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms.
It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 detected VEGFR2 phosphorylated on Tyr1054 in PAE cells transfected with CSF/KDR and treated with 40ng/mL CSF-1 for 15 min.
Cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P)
Optimal dilutions to be determined by the researcher.
Control:CSF-1 treated PAE cell lysate, transfected with CSF/KDR.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human VEGFR2 when phosphorylated on Tyrosine 1054. Species Crossreactivity: mouse, rat, primate, bovine, chicken, equine and zebrafish.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ch Eq Hu Mo Rt Ze
Immunogen：Synthetic peptide corresponding to VEGFR2 when phosphorylated on Tyrosine 1054.
