Rac is a member of the Rho family of small GTP-binding proteins.
It plays roles in cytoskeletal actin organization as well as transformation along with its other Rho family members.
Rac, Rho, and Cdc42 all work in concert with one another in the assembly of actin-based structures such as lamellipodia, stress fibers, and filopodia, respectively to control cell migration.
Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho.
Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases.
The latter pathway is activated by the PAK kinases, which are direct targets of Rac.
Rac1b is a highly active splice varient of Rac1.
It differs in that it has an additional 19aa.
It has been found in various tumors such as breasts and colorectal.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: ParaffinOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.07% sodium azide, 30% glycerol.
Specificity：Recognizes Rac1b. Species Crossreactivity: mouse and human.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to internal region of Rac1b.
