Nerve Growth Factor Receptor, also termed NGFR p75, p75NTR, or CD271 is the low affinity NGFR (LNGFR) which binds NGF and other neurotrophins including BDNF, NT3, NT4/5 with similar, low affinity.
NGFR p75 is a 75kD transmembrane glycoprotein (399aa) consisting of an extracellular domain (222aa) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22aa), and a cytoplasmic domain (155aa).
NGFR p75 is mainly expressed in Schwann cells and neurons and in a variety of non neuronal cells.
NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system.
NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS.
Applications:Suitable for use in Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:50 and 1:300Immunocytochemistry: 1:500Immunoprecipitation: 1:500-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes the extracellular domain of mouse NGF Receptor p75. Does not recognize rat p75 by Western blot.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP
Calc Crossreactivity：Mo
Immunogen：Extracellular fragment generated from sequences from the third exon of mouse p75, aa43-161. Species Sequence Homology: rat; 96%, human; 94%
