The Janus family of tyrosine kinases (JAK) includes JAK1, JAK2, JAK3 and Tyk2.
This family associates with a variety of cytokine receptors and is activated by ligand binding.
Upon ligand binding to cytokine receptors, JAKs phosphorylate themselves and their associated receptors.
This provides multiple binding sites for signaling proteins containing SH2 or other phospho-tyrosine-binding domains, including STATs, Shc, insulin receptor substrates and FAK.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Control:RAW264.7 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes the protein kinase 1 domain of mouse JAK2. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Linear peptide corresponding to the protein kinase 1 domain of mouse JAK2, conjugated to KLH.
