Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system.
GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals.
The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2.
The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene.
Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region.
Alternative splicing of this gene generates 4 transcript variants.
Applications:Suitable for use in Immunohistochemistry.
Although a band is observed around the calculated molecular weight of 105kD, Western Blotting is not a recommended application for this antibody because the band is weak in comparison to other non-specific bands present.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:300 detected GABA-B-R2 in Purkinje cells in rat mid-brain region
Optimal dilutions to be determined by the researcher.
Control:Purkinje cells in rat mid-brain region
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：guinea pig
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide.
Specificity：Recognizes the C-terminal domain of rat GABA-B-R2. Species Crossreactivity: human, bovine, equine, rhesus macaque, chimpanzee, mouse and canine.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Bo Ca Eq Hu Mc Mk Mo Rt
Immunogen：Linear peptide corresponding to the C-terminal domain of rat GABA-B-R2, conjugated to bovine thyroglobulin.
