Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport.
A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the aa identity, pharmacological specificity and effector responses.
DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4).
The two isoforms of D2R, D2 long (D2L) and short D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form.
It may play a role in the coupling of the receptor to G- proteins.
All members of this family have similar structure and contain 7 putative transmembrane domains.
A given cell or tissue may express more than one DR.
Specific radioligands do not exist that can differentiate between these DR.
Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.
Applications:Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1:100Western Blot: 1:500Immunohistochemistry: 1:5000 demonstrated staining of specific cells in various regions of rat brain sections.
Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb.
Optimal dilutions to be determined by the researcher.
Positive Control:Rat brain
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS, 10mg/ml BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. No preservatives added. Reconstitute with 100ul PBS, 10mg/ml BSA.
Specificity：Recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors. Species Crossreactivity: human.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide (ASLSQLSSHC) corresponding to aa2-10 of human dopamine D3 receptor.
