Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (Nimitrov 1997).
Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principle coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (Feng et al.
1996; Berson et al.
1996).
CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (Doranz et al.
1996; Endres et al.
1996).
The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (Bleul et al.
1996; Oberlin et al.
1996).
CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (Lapham et al.
1996).
CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (Feng et al.
1996).
Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (Feng et al.
1996; Endres et al.
1996; Brelot et al.
1997).
The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites (Brelot et al.
1997; Lu et al.
1997).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes the extracellular loop of human CXCR4. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to the extracellular loop of human CXCR4.
