Calnexin is a Ca++ binding protein found in ER transmembranes that binds to specific n-linked oligosaccharides found on newly synthesized proteins.
Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRu, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes.
Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:10,000Immunohistochemistry (Formalin fixed paraffin embedded): 1:1000-1:2000Immunocytochemistry: 1:500Optimal dilutions to be determined by the researcher.
Control:Human brain tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Calnexin. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Linear peptide corresponding to the C-terminal domain of human Calnexin, conjugated to KLH. Species sequence homology: chimpanzee, rhesus macaque, equine, porcine and bovine (100%).
