ATF6 (Activating Transcription Factor 6, Cyclic AMP-dependent transcription factor ATF6) is a stress activated leucine zipper transcription factor involved in activating transcription of endoplasmic reticulum (ER) molecules in response to accumulation of unfolded proteins in the ER.
ATF6 is originally a 90kD type II transmembrane glycoprotein that is converted to a 50kD soluble nuclear form, retaining its N-terminal domain, including the leucine zipper.
Unfolded protein accumulation in the ER results in the cleavage of ATF6 to release its cytoplasmic domain that then enters the nucleus.
ATF6 binds to an element designated ER Stress Response Element (ERSA) that appears in the promoter of glucose-regulated proteins (GRPs) such as GRP78, GPR94, and calreticulin.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control:HeLa nuclear lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes human ATF6.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to N-terminal domain of human ATF6, conjugated to KLH.
