Glucagon is a member of a multigene family comprising Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide. The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2
OXM, a 37aa peptide contains the glucagon sequence extended by a C-terminal basic octapeptide, its primary structure is identical in all mammals except in pig and cattle.
OXM is released from the gut during digestion, together with glicentin another octapeptide containing molecule.
It is considered as a putative physiological regulator of gastric acid secretion, it inhibits histamine.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL
ELISA: 0.5-1ug/ml Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:O8244-01: Oxyntomodulin, Human, Control Peptide (OXM)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. BSA Free
Specificity：Recognizes human OXM.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 9aa from the CT terminus of mature human Oxyntomodulin (OXM) conjugated to KLH. Species Sequence Homology: rat, mouse and guinea pig, 100%; bovine, porcine and sheep, 89%.
