Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography.
They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent.
The average molecular weight is reported to be about 160kD.
The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Applications:Suitable for use in Immunoelectrophoresis, Immunocytochemistry, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (IC): 1:20-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C.
Reconstitute with sterile ddH2O.
Do not freeze.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.01M Sodium Borate-Sodium Phosphate, 0.15M sodium chloride, pH 8.5, 15mg/ml BSA (IgG-Free, Protease-Free), 0.05% sodium azide. Labeled with 4nm Colloidal Gold. OD(520) = 2.0. Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes whole molecule human IgG. Reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Minimal cross-reactivity with bovine, equine and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
