A major nosocomial pathogen, Clostridium difficile causes antibiotic-associated colitis and intestinal Inflammatory disease, together called CDI.
By releasing two large protein enterotoxins, Toxin A (TcdA) and Toxin B (TcdB), Clostridium difficile mediates inflammatory diarrhea and compromises intestinal epithelial cells. Either toxin when administered to cultured cells disrupts the cytoskeleton and sets off capsase-dependent apoptosis, although, TcdB is many times more potent than TcdA. Both toxins also activate intestinal epithelial and immune cells to produce cytokines and chemokines, likely contributing to the complications associated with severe CDI.
Intracellular targets of TcdA and TcdB are RhoGTPases which regulate many host cell processes including establishing an epithelial barrier and the migration and signaling of immune cells.
When the Rho proteins are glycosylated by Toxins A or B, signaling is disrupted leading to the intestinal damage and inflamation characteristic of CDI.
Applications:Suitable for use in Western Blot, Capillary Precipitation and Double Immunodiffusion Assays.
Other applications not tested.
Recommended Dilution:Western Blot: 1:10 Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：goat
Grade：Serum
Purity：Serum
Form：Supplied as a sterile-filtered lyophilized powder. No preservative added. Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes Clostridium difficile Toxin A.
