Produced by Vibrio cholerae, cholera toxin (CT) is a multimeric enterotoxin that transfers ADP-ribose to a G protein, locking adenyl cyclase in an 'on' position.
By binding to the membrane of enteric cells, cholera toxin stimulates the cellular adenylate cyclase system, causing the hyper-secretion of chloride and bicarbonate ions, which results in increased fluid secretion and the severe diarrhea characteristic of the disease cholera.
The pentameric B subunit of CT binds with high efficiency to GM1 monosialoganglioside cell membrane receptors, present in many cell types, allowing its use experimentally in cell culture. Following binding, the toxin is endocytosed and travels retrogradely to the endoplasmic reticulum where the enzymatically active A subunit (CTA1) is translocated to the cytosol. Within the cytosol, CTA1 catalyzes the ADP-ribosylation of the Gs protein, activating adenylate cyclase and as a consequence increasing the intracellular concentration of cAMP. Relevant to the development of vaccine adjuvants, CT effectively modulates mammalian immune systems and acts as an adjuvant for co-administered antigens.
Applications:Suitable for use in Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at 4°C.
Reconstituted product is stable for 6 months at 4°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：goat
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in 0.09% sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes the B subunit of Cholera toxin.
