Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification.
Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Immunohistochemistry(Paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:Recognizes human ZIC3. Does not cross-react with other ZIC proteins. Species Crossreactivity: Bovine, Canine, Guinea pig, Gorilla, Equine, Monkey, Mouse, Orangutan, Rabbit, Rat.
Isotype:IgG
Calc Applications Abbrev:E IHC
Calc Crossreactivity:Bo Ca Eq Gp Hu Mk Mo Rb Rt
Immunogen:Synthetic peptide corresponding to aa178-189, C-HVDNNQVHLGLR, from an internal region of human ZIC3 (NP_003404.1).