Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids.
Major substrate is lysophosphatidylcholine.
Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility.
Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP.
Involved in several motility-related processes such as angiogenesis and neurite outgrowth.
Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation.
Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.
May have a role in induction of parturition.
Possible involvement in cell proliferation and adipose tissue development.
Tumor cell motility-stimulating factor.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.3-1ug/mlImmunohistochemistry(Paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human ENPP2. This antibody is expected to recognize all reported isoforms (NP_006200.3; NP_001035181.1, NP_001124335.1). Species Crossreactivity: Chimpanzee, Monkey.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to PDHLTSCVRPDVRVS from an internal region of human ENPP2 / Autotaxin (NP_006200.3, NP_001035181.1, NP_001124335.1).
