Vimentins are class-III intermediate filaments specific to mesenchymal tissue.
Vimentin is an important cytoskeletal component responsible for maintaining cell integrity and has a probable role in the intracellular transport of proteins such as lipoproteins between the nucleus and plasma membrane.
Immunohistochemical staining for Vimentin is characteristic of sarcomas.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000-1:10,000Immunocytochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：chicken
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.
Specificity：Recognizes human Vimentin. Species Crossreactivity: Mouse, rat.
Isotype：IgY
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human Vimentin purified from E. coli.
