This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.
The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000-1:10,000, detects ~24kD UCHL1 enzymeImmunocytochemistry: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Rat spinal cord or peripheral nerve homogenate
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：chicken
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.
Specificity：Recognizes human Ubiquitin C Terminal Hydrolase 1. Species Crossreactivity: Rat, mouse, bovine, porcine
Isotype：IgY
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Bo Hu Mo Po Rt
Immunogen：Recombinant full length protein corresponding to human Ubiquitin C-terminal Hydrolase 1, expressed in E. coli.
