84-2267-61　Tyrosine Hydroxylase (Tyrosine Hydroxylase, Tyrosine 3-monooxygenase, L-tyrosine Hydroxylase, Tyrosine 3-hydroxylase) 50ug　215500

特徴

• Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine.
• Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines.
• Plays an important role in the physiology of adrenergic neurons.
• CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin.
• COFACTOR: Fe(2+) ion.
• ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity.
• PATHWAY: Catecholamine biosynthesis; first step.
• SUBUNIT: Homotetramer.
• PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.
• SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
• The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia.
• Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (formalin fixed/paraffin embedded tissues): 0.5-1ug/ml.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.