84-2267-58　［取扱停止］Tumor Necrosis Factor, alpha (TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, Cachectin, TNF, TNFA, TNFSF2) 100ug　215497

特徴

• Tumor necrosis factor alpha (TNF-alpha) is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily.
• TNF-alpha is mainly secreted by macrophages.
• TNF-alpha is involved in the regulation of many processes such as  cell proliferation, differentiation and apoptosis and has been implicated in a variety of diseases including cancer.
• In microglia, TNF-alpha has been found to induce neurotoxicity via glutamate release.  TNF-alpha is expressed as a single-pass type II membrane protein.
• A soluble, secreted form is derived from the membrane form by proteolytic processing.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry (formalin fixed/paraffin embedded tissues): 1ug/ml.
• Heat mediated antigen retrieval is recommended
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.