Smad proteins are essential mediators of signal transduction by the TGF-beta superfamily from the cell surface into the nucleus.
Once in the nucleus, Smad proteins target a number of DNA binding proteins to stimulate transcription.
Smad4, also known as the common-mediator Smad (co-Smad), interacts with the receptor-regulated Smad proteins (R-SMAD).
The R-SMAD class includes Smad2 and Smad3.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-.05ug/mlImmunohistochemistry: 0.5-1ug/ml (formalin fixed/paraffin embedded tissues, formalin/acetone fixed tissues)Immunohistochemistry (Frozen): 0.4-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.9mg sodium chloride, 0.2mg Na2HPO4, 50% glycerol.
Specificity：Recognizes human Smad2/3. Predicted length of human Smad2 is 467aa and MW of 52kD. Predicted length of human Smad3 is 425aa and MW of 48kD.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to EQNGQEEKWCEKAVKS, from the N-terminal of human Smad2 and Smad3. Species Sequence Homology: Rat, mouse
