84-2267-39　［取扱停止］S100 Calcium-Binding Protein B (Protein S100-B, S100B, Calcium Binding Protein B1, S-100 Protein Beta Chain, S-100 Protein Subunit Beta) 100ug　215458

特徴

• Protein S100 beta belongs to the S100 family of proteins containing 2 EF-hand calcium binding motifs.
• S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation.
• Among brain cells, S100 beta is often used as a marker for astrocytes.
• The 2 subunits of S100 protein are the products of separate genes.
• The beta subunit is present in all S100 positive cells and tumors whereas the alpha subunit is detectable only in specific tissues such as heart, skeletal muscle and brain.
• The S100 protein is a homodimer composed of two alpha subunits (S100a) or two beta subunits (S100b) or as a heterodimer with one alpha and beta subunit (S100ab or S100ao).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry (Paraffin): 1ug/ml.
• Heat mediated antigen retrieval is recommended
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.