Runt-related transcription factor 2 (RUNX2) is a member of the RUNX family of transcription factors.
RUNX2 has an Runt domain and can bind DNA both as a monomer or as a subunit of a heterodimeric complex.
RUNX2 has a role in osteoblastic differentiation and skeletal morphogenesis and is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification.
RUNX2 is specifically expressed in the nucleus of osteoblasts.
3 isoforms are produced by alternative splicing.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human Runt-related Transcription Factor 2. Species Crossreactivity: Rat. Predicted length of 521 residues and MW of 57kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa244-258 from human Runt-related Transcription Factor 2, coupled to carrier protein BSA. Species Sequence Homology: Mouse
