Serine/threonine-protein phosphatase 2A is one of four major serine and threonine phosphatases and plays an important role in processes such as the regulation of the cell cycle, cell growth and division.
It has a common heteromeric core enzyme composed of a catalytic subunit and a constant regulatory subunit.
PP2A alpha is the alpha isoform of the catalytic subunit.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (formalin fixed/paraffin embedded tissues): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human PP2A Alpha. Species Crossreactivity: Mouse, rat, rabbit. Predicted length of 309 residues and MW of 36kD.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rb Rt
Immunogen：Synthetic peptide corresponding to aa6-20, FTKELDQWIEQLNEC, from human PP2A Alpha isoform, coupled to carrier protein BSA.
