The enzyme phosphomevalonate kinase (PMVK) catalyzes the conversion of 5-phosphomevalonate into 5-diphosphomevalonate in the cholesterol biosynthetic pathway.
PMVK is found in the peroxisome of heart, liver, skeletal muscle, kidney and pancreas tissue with lower levels in brain, placenta and lung.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (formalin fixed/paraffin embedded tissues): 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human Phosphomevalonate Kinase. Species Crossreactivity: Mouse, rat. Predicted length of 192 residues and MW of 22kD.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa135-151, EQSRQQRGWVFTPGVDD, from human Phosphomevalonate Kinase, coupled to carrier protein BSA.
