Enolase is a metalloenzyme that catayzes the reaction between 2-phospho-D-glycerate and phosphoenolpyruvate during glycolysis.
Mammalian enolase is composed of 3 subunits; alpha, beta and gamma (Neuron-specific enolase).
These subunits can form homodimers or heterodimers.
The alpha/gamma heterodimer and the gamma/gamma homodimer are found primarily in neurons.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human Neuron-specific Enolase. Species Crossreactivity: Mouse, rat. Predicted length of 434 residues and MW of 47kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa420-434, EARFAGHNFRNPSVL, from the C-terminal of human Neuron-specific Enolase, coupled to carrier protein BSA.
