Neurofilaments are composed of three intermediate filament proteins: light (~68kD), medium (~160kD) and heavy (~200kD), which are involved in the maintenance of the neuronal caliber.
Neurofilament medium runs on SDS-PAGE gels in the range 145-170kD, with some variation in different species.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000-1:10,000Immunocytochemistry: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：chicken
Source Antibody：rat
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.
Specificity：Recognizes rat Neurofilament Medium. Species Crossreactivity: Human, mouse feline, chicken
Isotype：IgY
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Ch Fe Hu Mo Rt
Immunogen：Recombinant fusion protein corresponding to extreme C-terminal segment of rat Neurofilament Medium.
