Neurofilaments are composed of three intermediate filament proteins: light (~68kD), medium (~160kD) and heavy (~200kD), which are involved in the maintenance of the neuronal caliber.
Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000-1:10,000Immunocytochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：chicken
Source Antibody：porcine
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.
Specificity：Recognizes porcine Neurofilament Light. Species Crossreactivity: Human, rat, mouse, feline, chicken.
Isotype：IgY
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Ch Fe Hu Mo Po Rt
Immunogen：Purified porcine NF-L from spinal cord and recombinant NF-L.
