Neurofilaments contain three intermediate filament proteins: light (68kD), medium (160kD) and heavy (200kD).
Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Applications:Suitable for use in Western Blot, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000-1:10,000Immunocytochemistry: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with sterile ddH2O.
Specificity：Recognizes phosphorylated and non-phosphorylated rat Neurofilament Heavy.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Rt
Immunogen：Purified rat NF-H construct containing most of the tandem KSP repeats expressed and purified from E. coli.
