Neurofilaments contain three intermediate filament proteins: light (68kD), medium (160kD) and heavy (200kD).
Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
This antibody binds primarily to the phosphorylated axonal forms of NF-H.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:100,000-1:1,000,000Immunohistochemistry: 1:50,000-1:100,000Immunocytochemistry: 1:50,000-1:100,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：chicken
Source Antibody：bovine
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder, pH 7.4. Reconstitute with 50ul sterile ddH2O.
Specificity：Recognizes bovine Neurofilament Heavy Phosphorylated. Species Crossreactivity: human, rat, and mouse
