Part of the host defense system of polymorphonuclear leukocytes.
It is responsible for microbicidal activity against a wide range of organisms.
In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms.
COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer.
SUBUNIT: Tetramer of two light chains and two heavy chains.
ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing.
DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD).
MPD is an autosomal recessive defect that results in disseminated candidiasis.
SIMILARITY: Belongs to the peroxidase family.
XPO subfamily.
Microglia and astrocytes are known to express MPO as well.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (formalin fixed/paraffin embedded tissues): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human Myeloperoxidase. Species Crossreactivity: Mouse, rat. Predicted length of 745 residues and MW of 84kD.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa714-728, KNNIFMSNSYPRDFV, from human Myeloperoxidase, coupled to carrier protein BSA. Cellular Localization: Lysosome.
