84-2266-72　Microtubule Associated Protein 2 (MAP2, MAP-2) 50ul　215363

特徴

• Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells.
• They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit.
• Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5.
• MAP2 is made up of two ~280kD apparent molecular weight bands referred to as MAP2a and MAP2b.
• A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kD on SDS-PAGE gels.
• All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau).
• MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells.
• Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
• Applications:Suitable for use in Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry: 1:2500-1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.