84-2266-59 [取扱停止]Lamin B1 (LMNB1, LMN2, LMNB) 100ug 215349
特徴
- Lamins belong to the intermediate filament family of proteins.
- They are structural components of the nuclear lamina and are thought to be involved in nuclear stability, chromatin structure and gene expression.
- There are two main types of lamins; A and B.
- The A type lamins include A, C and Adel10.
- The B-type lamins, B1 and B2, arise from two distinct genes.
- Applications:Suitable for use in Western Blot and Immunohistochemistry.
- Other applications not tested.
- Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry:1ug/ml (formalin fixed/paraffin embedded tissues, formalin/acetone fixed tissues).
- Antigen retrieval by Pepsin and Trypsin is required in acetone fixed cells
- Optimal dilutions to be determined by the researcher.
- Storage and Stability:Lyophilized powder may be stored at -20°C.
- Stable for 12 months after receipt at -20°C.
- Reconstitute with sterile ddH2O.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Reconstituted product is stable for 12 months at -20°C.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Further dilutions can be made in assay buffer.
仕様
- Size:100ug
- Host:rabbit
- Source Antibody:human
- Grade:Affinity Purified
- Purity:Purified by immunoaffinity chromatography.
- Form:Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.
- Specificity:Recognizes human Lamin B1. Species Crossreactivity: Mouse, rat. Predicted length of 586 residues and MW of 66kD.
- Isotype:IgG
- Calc Applications Abbrev:IHC WB
- Calc Crossreactivity:Hu Mo Rt
- Immunogen:Synthetic peptide corresponding to aa570-586, FHQQGTPRASNRSCAIM, from human Lamin B1, coupled to carrier protein BSA.
- Shelf Life:1year
- Swiss Prot Number:P20700
- EU Commodity Code:38221010
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