84-2266-57　Internexin, alpha (67kD Neurofilament Protein, NF-66, Neurofilament-66, Alpha-Internexin, Alpha-Inx, Neurofilament 5, INA, NEF5) 50ul　215345

特徴

• Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells.
• They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H.
• Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin.
• Alpha-internexin is a ~66kD Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes.
• Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H.
• In some mature neurons alpha-internexin is the only neurofilament subunit expressed.
• Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture.
• In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
• Applications:Suitable for use in Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:10,000-1:20,000 Immunocytochemistry: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.