The High Mobility Group (HMG)-box conserved domain is found in proteins that promote chromatin function and gene regulation, assist with transcription, replication, recombination and DNA repair.
High mobility group protein B4 (HMGB4) contains two HMG-box DNA-binding domains.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human High Mobility Group Protein B4. Species Crossreactivity: Mouse, rat. Predicted length of 186 residues and MW of 22kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to, RNKFKEQQPNTYVGFKEF which differs by a single residue to aa24-41 from human High Mobility Group Protein B4, coupled to carrier protein BSA.
